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rabbit anti phospho mtor ser2448 cell signaling technology  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti phospho mtor ser2448 cell signaling technology
    Rabbit Anti Phospho Mtor Ser2448 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 6895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pan+mtor/Akt+(pan)+Rabbit+mAb/pm41168352-222-33-36
    Average 98 stars, based on 6895 article reviews
    rabbit anti phospho mtor ser2448 cell signaling technology - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Elevated expression of HSP90 and the antitumor effect of an HSP90 inhibitor via inactivation of the Akt/mTOR pathway in undifferentiated pleomorphic sarcoma
    Article Snippet: .. Protein was extracted from five available frozen samples paired with normal muscular tissue and from the cultured UPS cells after they were treated with 17-DMAG (0.3 μmol/L) for 6, 12 or 24 h. In addition to the antibodies used for the IHC analysis, the rabbit antibodies for pan-Akt (C67E7; 1:400 dilution), pan-mTOR (1:400 dilution), and pan-S6RP (5G10; 1:400 dilution) (Cell Signaling Technology) were used as the primary antibody. .. For an internal control, an anti-glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) (1:5,000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) mouse monoclonal antibody was used.

    Immunohistochemistry:

    Article Title: Elevated expression of HSP90 and the antitumor effect of an HSP90 inhibitor via inactivation of the Akt/mTOR pathway in undifferentiated pleomorphic sarcoma
    Article Snippet: .. Protein was extracted from five available frozen samples paired with normal muscular tissue and from the cultured UPS cells after they were treated with 17-DMAG (0.3 μmol/L) for 6, 12 or 24 h. In addition to the antibodies used for the IHC analysis, the rabbit antibodies for pan-Akt (C67E7; 1:400 dilution), pan-mTOR (1:400 dilution), and pan-S6RP (5G10; 1:400 dilution) (Cell Signaling Technology) were used as the primary antibody. .. For an internal control, an anti-glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) (1:5,000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) mouse monoclonal antibody was used.

    Incubation:

    Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development.
    Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), pan-mTOR (CST, 2983T) and phospho-mTOR (Ser2448; CST, 5536T) in blocking solution (1% bovine serum albumin in PBS) overnight at 4 ̊C. ..

    Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development
    Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), pan-mTOR (CST, 2983T) and phospho-mTOR (Ser2448; CST, 5536T) in blocking solution (1% bovine serum albumin in PBS) overnight at 4°C. ..

    Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cells development
    Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated SuperfrostTM Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), pan-mTOR (CST, 2983T) and phospho-mTOR (Ser2448; CST, 5536T) in blocking solution (1% bovine serum albumin in PBS) overnight at 4°C. ..

    Blocking Assay:

    Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development.
    Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), pan-mTOR (CST, 2983T) and phospho-mTOR (Ser2448; CST, 5536T) in blocking solution (1% bovine serum albumin in PBS) overnight at 4 ̊C. ..

    Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development
    Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), pan-mTOR (CST, 2983T) and phospho-mTOR (Ser2448; CST, 5536T) in blocking solution (1% bovine serum albumin in PBS) overnight at 4°C. ..

    Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cells development
    Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated SuperfrostTM Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), pan-mTOR (CST, 2983T) and phospho-mTOR (Ser2448; CST, 5536T) in blocking solution (1% bovine serum albumin in PBS) overnight at 4°C. ..

    Phospho-proteomics:

    Article Title: Inhibition of epithelial-mesenchymal transition in bladder cancer cells via modulation of mTOR signalling.
    Article Snippet: Mounting evidence suggests that signalling crosstalk plays a significant role in the regulation of epithelial–mesenchymal transition (EMT) in cancer cells.. However, the complex network regulating the EMT in different cancer types has not been fully described yet which affects the development of novel therapeutic strategies.. In the present study, we investigated the signalling pathways involved in EMT of bladder cancer cells and demonstrated the effects of two novel agents in the regulation of EMT.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Neuroprotective Impact of Linagliptin against Cadmium-Induced Cognitive Impairment and Neuropathological Aberrations: Targeting SIRT1/Nrf2 Axis, Apoptosis, and Autophagy
    Article Snippet: RayBiotech ELISA kit was used for the quantification of the phosphorylated and total forms of AMPK under the guidance of the vendor (Cat. # PEL-AMPKA-S487-T; Norcross, GA, USA). .. Likewise, ELISA kits from Cell Signaling were used to quantify phosphorylated and total forms of mTOR (Cat. # 7976C for p-mTOR[Ser2448] and Cat. # 7974C for pan mTOR, Cell Signaling Technology, Danvers, MA, USA). ..



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    Salmonella modulation of gallbladder epithelial cell molecular pathways. Epithelial cells from HODGM model were exposed to 13 S . Typhi strains isolated from acutely infected (n=6, ) or chronically (n=7, ) infected individuals. HODGM models cultured with media only were used as negative controls. After 5 hours, the cells were harvested, lysed, and phosphorylation of (A) AKT, (B) YAP, (C) <t>mTOR,</t> (D) MAPK, and (E) S6 were detected by western blot. Data are representative of the net responses observed in at least 4 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed to S . Typhi. Each dot is the average of 2 technical independent replicates. The density was normalized to the corresponding pan kinase. Two-tailed nested-t-tests were used to account for the repeated measures within the groups. Dotted lines represent the mean responses. (F) Shown is a representative immunoblot analysis of protein lysates. Results from uninfected media controls (UN) and acute and chronic strains are displayed (G) Correlation between phosphorylation levels of S6 and MAPK using the combined data after exposure to S. Typhi strains isolated from acutely and chronically infected individuals. The solid line represents the trendline. Dashed lines represent 95% confidence intervals. Shown are the coefficient of determination “r” and the “ P ” value. Correlations used the two-sided Pearson Product Moment tests. (H-J) MAPK regulated transcription factors (H) Phospho-ATF-2, (I) Phospho-MEF2, and (J) Phospho-c-Myc were detected by ELISA-based assays, TransAM assays. Data are representative of the net responses observed in 5 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed S . Typhi. Each dot is the average of 2 technical independent replicates. Two-tailed nested-t-test was used to account for the repeated measures within the groups. P values < 0.05 were considered statistically significant. Dotted lines represent the mean responses.
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    Image Search Results


    Salmonella modulation of gallbladder epithelial cell molecular pathways. Epithelial cells from HODGM model were exposed to 13 S . Typhi strains isolated from acutely infected (n=6, ) or chronically (n=7, ) infected individuals. HODGM models cultured with media only were used as negative controls. After 5 hours, the cells were harvested, lysed, and phosphorylation of (A) AKT, (B) YAP, (C) mTOR, (D) MAPK, and (E) S6 were detected by western blot. Data are representative of the net responses observed in at least 4 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed to S . Typhi. Each dot is the average of 2 technical independent replicates. The density was normalized to the corresponding pan kinase. Two-tailed nested-t-tests were used to account for the repeated measures within the groups. Dotted lines represent the mean responses. (F) Shown is a representative immunoblot analysis of protein lysates. Results from uninfected media controls (UN) and acute and chronic strains are displayed (G) Correlation between phosphorylation levels of S6 and MAPK using the combined data after exposure to S. Typhi strains isolated from acutely and chronically infected individuals. The solid line represents the trendline. Dashed lines represent 95% confidence intervals. Shown are the coefficient of determination “r” and the “ P ” value. Correlations used the two-sided Pearson Product Moment tests. (H-J) MAPK regulated transcription factors (H) Phospho-ATF-2, (I) Phospho-MEF2, and (J) Phospho-c-Myc were detected by ELISA-based assays, TransAM assays. Data are representative of the net responses observed in 5 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed S . Typhi. Each dot is the average of 2 technical independent replicates. Two-tailed nested-t-test was used to account for the repeated measures within the groups. P values < 0.05 were considered statistically significant. Dotted lines represent the mean responses.

    Journal: Frontiers in Immunology

    Article Title: Early host immune responses in a human organoid-derived gallbladder monolayer to Salmonella Typhi strains from patients with acute and chronic infections: a comparative analysis

    doi: 10.3389/fimmu.2024.1334762

    Figure Lengend Snippet: Salmonella modulation of gallbladder epithelial cell molecular pathways. Epithelial cells from HODGM model were exposed to 13 S . Typhi strains isolated from acutely infected (n=6, ) or chronically (n=7, ) infected individuals. HODGM models cultured with media only were used as negative controls. After 5 hours, the cells were harvested, lysed, and phosphorylation of (A) AKT, (B) YAP, (C) mTOR, (D) MAPK, and (E) S6 were detected by western blot. Data are representative of the net responses observed in at least 4 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed to S . Typhi. Each dot is the average of 2 technical independent replicates. The density was normalized to the corresponding pan kinase. Two-tailed nested-t-tests were used to account for the repeated measures within the groups. Dotted lines represent the mean responses. (F) Shown is a representative immunoblot analysis of protein lysates. Results from uninfected media controls (UN) and acute and chronic strains are displayed (G) Correlation between phosphorylation levels of S6 and MAPK using the combined data after exposure to S. Typhi strains isolated from acutely and chronically infected individuals. The solid line represents the trendline. Dashed lines represent 95% confidence intervals. Shown are the coefficient of determination “r” and the “ P ” value. Correlations used the two-sided Pearson Product Moment tests. (H-J) MAPK regulated transcription factors (H) Phospho-ATF-2, (I) Phospho-MEF2, and (J) Phospho-c-Myc were detected by ELISA-based assays, TransAM assays. Data are representative of the net responses observed in 5 experiments. Net responses were calculated by subtracting the responses of the controls (media) from those in cells exposed S . Typhi. Each dot is the average of 2 technical independent replicates. Two-tailed nested-t-test was used to account for the repeated measures within the groups. P values < 0.05 were considered statistically significant. Dotted lines represent the mean responses.

    Article Snippet: The following primary anti-human antibodies were used for Western Blot: (1) rabbit anti-phospho S6 (Ser240/244) mAb (1:2000) (clone D68F8), (2) rabbit anti-pan S6 mAb (1:1000) (clone 5G10), (3) rabbit anti-phospho MAPK(p-p44/42 extracellular signal-regulated kinase (ERK) 1/2) mAb (1:1000) (clone D13.14.4E), (4) rabbit anti-pan MAPK(p-p44/42 Erk1/2) polyclonal antibody (1:1000), (5) rabbit anti-phospho YAP (Ser109) mAb (1:600) (clone E5I9G), (6) rabbit anti-pan YAP mAb (1:1000) (clone D8H1X), (7) rabbit anti-phospho Axl (Tyr702) mAb (1:1000) (clone D12B2), (8) rabbit anti-pan Axl mAb (1:1000) (clone C44G1), (9) rabbit anti-phospho Akt (Ser473) mAb (1:2000) (clone D9E), (10) rabbit anti-pan Akt mAb (1:1000) (clone C67E7), (11) rabbit anti-phospho mTOR (Ser2448) polyclonal antibody (1:1000), (12) rabbit anti-pan mTOR polyclonal antibody (1:1000), (13) rabbit anti- Enhancer of zeste homolog 2 (EZH2) mAb (1:1000) (clone D2C9), (Cell Signaling, Danvers, MA, USA), (14) mouse anti-interferon regulatory factor 1 (IRF1) mAb (1:500) (clone 686703), and (15) rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) polyclonal antibody (1:15000) (R&D Systems, Minneapolis, MN).

    Techniques: Isolation, Infection, Cell Culture, Phospho-proteomics, Western Blot, Two Tailed Test, Enzyme-linked Immunosorbent Assay