rabbit anti phospho mtor ser2448 cell signaling technology (Cell Signaling Technology Inc)
Structured Review
Rabbit Anti Phospho Mtor Ser2448 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 6895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pan+mtor/Akt+(pan)+Rabbit+mAb/pm41168352-222-33-36
Average 98 stars, based on 6895 article reviews
Images
Related Articles
Cell Culture:Article Title: Elevated expression of HSP90 and the antitumor effect of an HSP90 inhibitor via inactivation of the Akt/mTOR pathway in undifferentiated pleomorphic sarcoma Article Snippet: .. Protein was extracted from five available frozen samples paired with normal muscular tissue and from the cultured UPS cells after they were treated with 17-DMAG (0.3 μmol/L) for 6, 12 or 24 h. In addition to the antibodies used for the IHC analysis, the rabbit antibodies for pan-Akt (C67E7; 1:400 dilution), Immunohistochemistry:Article Title: Elevated expression of HSP90 and the antitumor effect of an HSP90 inhibitor via inactivation of the Akt/mTOR pathway in undifferentiated pleomorphic sarcoma Article Snippet: .. Protein was extracted from five available frozen samples paired with normal muscular tissue and from the cultured UPS cells after they were treated with 17-DMAG (0.3 μmol/L) for 6, 12 or 24 h. In addition to the antibodies used for the IHC analysis, the rabbit antibodies for pan-Akt (C67E7; 1:400 dilution), Incubation:Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development. Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cells development Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated SuperfrostTM Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), Blocking Assay:Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development. Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated Superfrost Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), Article Title: CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cells development Article Snippet: For IF staining of hPGCLCs, day 4 aggregates were dissociated into single cells and spread onto poly-L-lysine-treated SuperfrostTM Plus Microscope Slides (Fisher Scientific). .. The slides were fixed in 4% paraformaldehyde in PBS for 15 min at room temperature (RT), washed three times with PBS for 5 min, permeabilized with 0.2% Triton-X in PBS for 10 min at RT and washed three times with PBS for 5 min. Next, the slides were incubated with primary antibodies to pan-AKT (CST, 4691T), phospho-AKT (Ser473; CST, 4060T), Phospho-proteomics:Article Title: Inhibition of epithelial-mesenchymal transition in bladder cancer cells via modulation of mTOR signalling. Article Snippet: Mounting evidence suggests that signalling crosstalk plays a significant role in the regulation of epithelial–mesenchymal transition (EMT) in cancer cells.. However, the complex network regulating the EMT in different cancer types has not been fully described yet which affects the development of novel therapeutic strategies.. In the present study, we investigated the signalling pathways involved in EMT of bladder cancer cells and demonstrated the effects of two novel agents in the regulation of EMT. Enzyme-linked Immunosorbent Assay:Article Title: Neuroprotective Impact of Linagliptin against Cadmium-Induced Cognitive Impairment and Neuropathological Aberrations: Targeting SIRT1/Nrf2 Axis, Apoptosis, and Autophagy Article Snippet: RayBiotech ELISA kit was used for the quantification of the phosphorylated and total forms of AMPK under the guidance of the vendor (Cat. # PEL-AMPKA-S487-T; Norcross, GA, USA). .. Likewise, ELISA kits from Cell Signaling were used to quantify phosphorylated and total forms of mTOR (Cat. # 7976C for p-mTOR[Ser2448] and Cat. # |
